Journal: Reproductive Sciences

Article Title: The Essential Role of GATA6 in the Activation of Estrogen Synthesis in Endometriosis

doi: 10.1177/1933719118756751

Figure Lengend Snippet: A, Differences in estradiol (E2) production in OSIS experimental groups. N = 4. Asterisk (*) indicates significantly lower concentration compared to other groups tested (P < .05). B, Differences in estradiol (E2) production in normal endometrial stromal cells (NoEMs) experimental groups. N = 4. Asterisk (*) indicates a significantly higher concentration compared to other groups tested (P < .05). C, Differences in estradiol (E2) production in skin fibroblast (BJ) experimental groups. N = 4.

Article Snippet: Human foreskin fibroblast cells (BJ cells), a nonimmortalized stromal cell line with a normal diploid karyotype, were obtained from ATCC and used as controls (cat. CRL-2522; Manassas, Virginia; n = 7).

Techniques: Concentration Assay

Journal: eLife

Article Title: The spatial separation of processing and transport functions to the interior and periphery of the Golgi stack

doi: 10.7554/eLife.41301

Figure Lengend Snippet:

Article Snippet: Cell line ( Rattus norvegicus ) , Normal rat kidney (NRK) fibroblast cell , ATCC , ATCC: CRL-1570; RRID: CVCL_2144 , .

Techniques: Recombinant, Plasmid Preparation, Antibody Labeling, Software

Journal: Scientific Reports

Article Title: Chemical synthesis, bioinformatics and antiproliferative studies of novel pyrimidin-4-yl-1H-pyrazole derivatives in chronic myeloid cancer cells

doi: 10.1038/s41598-025-26292-7

Figure Lengend Snippet: Cytotoxicity of compounds in CML imatinib sensitive (K562) and resistant (K562-IM) cell lines and normal dermal fibroblasts after 72 h incubation. Cytotoxicity of compounds 2 , 2a , 2b , 2c , 3 , 3a , 4 and 7 in ( A ) K562 cell line and ( B ) K562-IM cell line. Cytotoxicity of compounds 5 and 6 in ( C ) K562 cell line, ( D ) K562-IM cell line and ( E ) normal dermal fibroblasts. * p value < 0.05 relative to respective cells treated with DMSO.

Article Snippet: Human Primary dermal fibroblasts (PCS-201-010, ATCC) were also used as normal control cells.

Techniques: Incubation

Journal: International Journal of Molecular Sciences

Article Title: Functionalization of Bacterial Cellulose with the Antimicrobial Peptide KR-12 via Chimerical Cellulose-Binding Peptides

doi: 10.3390/ijms25031462

Figure Lengend Snippet: Solution peptide effect on NHDF viability assessed through metabolic activity. All data are normalized to the metabolic activity of untreated control cells indicated by the red dashed line; 70% activity is indicated by the grey dashed line. Data are presented as the mean and standard deviation from three independent experiments ( n = 3 per experiment). Statistical differences determined by an ANOVA followed by the Tukey’s HSD test (* p < 0.05, ** p < 0.01, *** p < 0.005).

Article Snippet: Komagataeibacter hansenii NQ5 ( K. hansenii ATCC 53582), normal human dermal fibroblasts (NHDFs; American Type Culture Collection: CRL-2565), Staphylococcus aureus (ATCC 43866, strain designation: LRA 44.01.83), Escherichia coli (ATCC 33694, strain designation: HB101) and Pseudomonas aeruginosa (ATCC 29260, strain designation: PA-103) were purchased from the American Type Culture Collection (ATCC), Manassas, VA, USA, while human endothelial keratinocyte cells (HaCaT, catalog number: T0020001) were purchased from AddexBio, San Diego, CA, USA.

Techniques: Activity Assay, Control, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Functionalization of Bacterial Cellulose with the Antimicrobial Peptide KR-12 via Chimerical Cellulose-Binding Peptides

doi: 10.3390/ijms25031462

Figure Lengend Snippet: Functionalized BC effect on NHDF and HaCaT viability. Normalized metabolic activity of ( A , B ) NHDF and ( C , D ) HaCaT after exposure to unfunctionalized BC (BC control) and BC functionalized with Long-CBP-KR12 and Short-CBP-KR12 normalized to untreated cells. Both the ( A , C ) opaque and ( B , D ) transparent BC were evaluated. All data are normalized to the metabolic activity of untreated control wells indicated by the red dashed line, and 70% activity is indicated by the grey dashed line. Data are presented as the mean and standard deviation from three independent experiments ( n = 3 per experiment). No statistical differences determined by ANOVA.

Article Snippet: Komagataeibacter hansenii NQ5 ( K. hansenii ATCC 53582), normal human dermal fibroblasts (NHDFs; American Type Culture Collection: CRL-2565), Staphylococcus aureus (ATCC 43866, strain designation: LRA 44.01.83), Escherichia coli (ATCC 33694, strain designation: HB101) and Pseudomonas aeruginosa (ATCC 29260, strain designation: PA-103) were purchased from the American Type Culture Collection (ATCC), Manassas, VA, USA, while human endothelial keratinocyte cells (HaCaT, catalog number: T0020001) were purchased from AddexBio, San Diego, CA, USA.

Techniques: Activity Assay, Control, Standard Deviation

Journal: Nutrients

Article Title: Essential Oils, Pituranthos chloranthus and Teucrium ramosissimum , Chemosensitize Resistant Human Uterine Sarcoma MES-SA/Dx5 Cells to Doxorubicin by Inducing Apoptosis and Targeting P-Glycoprotein

doi: 10.3390/nu13051719

Figure Lengend Snippet: Effects of Pituranthos chloranthus (PC) and Teucrium ramosissimum Desf. (TR) extracts on normal primary human uterine fibroblast cells (HUF) and primary murine Bone Marrow-Derived Macrophages (BMDM) viability. After treatment of primary HUF and murine BMDM with increasing concentrations (0–100 µg/mL) of PC and TR for 72 h, the percentage of viable cells was assessed using the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. ( A ) Dose–response curves of PC-treated HUF (left panel) and TR-treated HUF (right panel). ( B ) Dose–response curves of PC-treated BMDM (left panel) and TR-treated BMDM (right panel). Data are expressed as a mean percentage of control growth ± Standard Deviation (SD) of two representative experiments ( n = 6 replicates per concentration).

Article Snippet: Primary human uterine fibroblast normal cells (HUF) were obtained from the ATCC.

Techniques: Derivative Assay, MTT Assay, Control, Standard Deviation, Concentration Assay